K1-0668
Bambanker™ Direct Cell Freezing Media

Bambanker Direct is a novel serum-free cell freezing medium, which can be added directly to the culture medium without centrifuging the collected cells. Then after resuspending the cells, they can then be frozen at -80 °C. This is recommended for Hybridoma cells and fragile cells where centrifugation can be a problem. Bambanker Direct is a novel serum-free cell freezing medium, which can be added directly to the culture medium without centrifuging the collected cells. Then after resuspending the cells, they can then be frozen at -80 °C. This is recommended for hybridomas and other fragile cell lines.  

Bambanker Direct for hybridomas

There are certain cell types, such as hybridomas, which exhibit increased sensitivity to “over” manipulations. These cells often exhibit higher death rates or unwanted differentiation after long-term storage and freeze thaw cycling. It is for these types of cells that Bambanker^TM Direct was created – to provide tight environmental control. By eliminating the centrifugation step, cells can now be frozen for long-term storage immediately upon addition of the novel cryoprotectant. Just add Bambanker^TM Direct, one-to one, with your cells in media and directly place in freezer. And like with the original Bambanker^TM, the Direct version has no serum, so it is great for cells which animal-derived serum may be an issue.   Procedure for the cryopreservation of cells